Saliva samples are a viable alternative to blood samples as a source of DNA for high throughput genotyping
نویسندگان
چکیده
منابع مشابه
Feasibility of High-Throughput Genome-Wide Genotyping using DNA from Stored Buccal Cell Samples
It is unclear if buccal cell samples contain sufficient human DNA with adequately sized fragments for high throughput genetic bioassays. Yet buccal cell sample collection is an attractive alternative to gathering blood samples for genetic epidemiologists engaged in large-scale genetic biomarker studies. We assessed the genotyping efficiency (GE) and genotyping concordance (GC) of buccal cell DN...
متن کاملAssessment of DNA Extracted from Forensic Samples Prior to Genotyping.
Quantification of human DNA has been an integral part of forensic DNA analysis. Hybridizationbased methods such as Quantiblot® kits were used extensively in the 1990s. These methods fulfilled the need at the time, since their sensitivity range was similar to the genotyping methods in use, such as restricted fragment length polymorphism. Later, the development of robust and more sensitive megapl...
متن کاملExtraction of DNA from Forensic Biological Samples for Genotyping.
Biological forensic samples constitute evidence with probative organic matter. Evidence believed to contain DNA is typically processed for extraction and purification of its nucleic acid content. Forensic DNA samples are composed of two things, a tissue and the substrate it resides on. Compositionally, a sample may contain almost anything and for each, the type, integrity, and content of both t...
متن کاملDevelopment of a high-throughput quantitative assay for detecting herpes simplex virus DNA in clinical samples.
We have developed a high-throughput, semiautomated, quantitative fluorescence-based PCR assay to detect and type herpes simplex virus (HSV) DNA in clinical samples. The detection assay, which uses primers to the type-common region of HSV glycoprotein B (gB), was linear from <10 to 10(8) copies of HSV DNA/20 microl of sample. Among duplicate samples in reproducibility runs, the assay showed less...
متن کاملDetection of Streptococcus mutans Genomic DNA in Human DNA Samples Extracted from Saliva and Blood
Caries is a multifactorial disease, and studies aiming to unravel the factors modulating its etiology must consider all known predisposing factors. One major factor is bacterial colonization, and Streptococcus mutans is the main microorganism associated with the initiation of the disease. In our studies, we have access to DNA samples extracted from human saliva and blood. In this report, we tes...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: BMC Medical Genomics
سال: 2012
ISSN: 1755-8794
DOI: 10.1186/1755-8794-5-19